Signalling

Part:BBa_K895003:Design

Designed by: Frank Sargent   Group: iGEM12_Dundee   (2012-09-22)

Salty_TtrRS_two_component_system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2550
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1179
    Illegal AgeI site found at 311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 28


Design Notes

A PstI site in the natural ttrS sequence has been silently removed. A BamHI site has been engineered at the extreme 3' end. This allows researchers to add a reporter gene of their choice at the correct distance from the RBS.

Source

PCR product using Salmonella LT2 genomic DNA as template.

References

Hensel,M., Hinsley,A.P., Nikolaus,T., Sawers,G. and Berks,B.C. (1999) The genetic basis of tetrathionate respiration in Salmonella typhimurium. Mol. Microbiol. 32:275-287.